Centimorgan-Range One-Step Mapping of Fertility Traits Using Interspecific Recombinant Congenic Mice

@article{citeulike:1474189, abstract = {In mammals, male fertility is a quantitative feature determined by numerous genes. Until now, several wide chromosomal regions involved in fertility have been defined by genetic mapping approaches; unfortunately, the underlying genes are very difficult to identify. Here, 53 interspecific recombinant congenic mouse strains (IRCSs) bearing 12\% SEG/Pas (Mus spretus) genomic fragments disseminated in a C57Bl/6J (Mus domesticus) background were used to systematically analyze male fertility parameters. One of the most prominent advantages of this model is the possibility of analyzing stable phenotypes in living animals. Here, we demonstrate the possibility in one-step fine mapping for several fertility traits. Focusing on strains harboring a unique spretus fragment, we could unambiguously localize two testis and one prostate weight-regulating QTL (Ltw1, Ltw2, and Lpw1), four QTL controlling the sperm nucleus shape (Sh1, Sh2, Sh3, and Sh4), and one QTL influencing sperm survival (Dss1). In several cases, the spretus DNA fragment was small enough to propose sound candidates. For instance, Spata1, Capza, and Tuba7 are very strong candidates for influencing the shape of the sperm head. Identifying new genes implied in mammalian fertility pathways is a necessary prerequisite for clarifying their molecular grounds and for proposing diagnostic tools for masculine infertilities. 10.1534/genetics.107.072157}, author = {L'Hote, David and Serres, Catherine and Laissue, Paul and Oulmouden, Ahmad and Rogel-Gaillard, Claire and Montagutelli, Xavier and Vaiman, Daniel }, citeulike-article-id = {1474189}, doi = {http://dx.doi.org/10.1534/genetics.107.072157}, journal = {Genetics}, keywords = {animal-genetics, animal-model, globozoospermia, male-fertility, sperm}, month = {July}, number = {3}, pages = {1907--1921}, posted-at = {2007-07-23 08:24:14}, priority = {2}, title = {Centimorgan-Range One-Step Mapping of Fertility Traits Using Interspecific Recombinant Congenic Mice}, url = {http://dx.doi.org/10.1534/genetics.107.072157}, volume = {176}, year = {2007} }

TY - JOUR ID - citeulike:1474189 L3 - citeulike-article-id:1474189 TI - Centimorgan-Range One-Step Mapping of Fertility Traits Using Interspecific Recombinant Congenic Mice JF - Genetics VL - 176 IS - 3 SP - 1907 EP - 1921 N2 - In mammals, male fertility is a quantitative feature determined by numerous genes. Until now, several wide chromosomal regions involved in fertility have been defined by genetic mapping approaches; unfortunately, the underlying genes are very difficult to identify. Here, 53 interspecific recombinant congenic mouse strains (IRCSs) bearing 12% SEG/Pas (Mus spretus) genomic fragments disseminated in a C57Bl/6J (Mus domesticus) background were used to systematically analyze male fertility parameters. One of the most prominent advantages of this model is the possibility of analyzing stable phenotypes in living animals. Here, we demonstrate the possibility in one-step fine mapping for several fertility traits. Focusing on strains harboring a unique spretus fragment, we could unambiguously localize two testis and one prostate weight-regulating QTL (Ltw1, Ltw2, and Lpw1), four QTL controlling the sperm nucleus shape (Sh1, Sh2, Sh3, and Sh4), and one QTL influencing sperm survival (Dss1). In several cases, the spretus DNA fragment was small enough to propose sound candidates. For instance, Spata1, Capza, and Tuba7 are very strong candidates for influencing the shape of the sperm head. Identifying new genes implied in mammalian fertility pathways is a necessary prerequisite for clarifying their molecular grounds and for proposing diagnostic tools for masculine infertilities. 10.1534/genetics.107.072157 KW - animal-genetics KW - animal-model KW - globozoospermia KW - male-fertility KW - sperm AU - L'Hote, David AU - Serres, Catherine AU - Laissue, Paul AU - Oulmouden, Ahmad AU - Rogel-Gaillard, Claire AU - Montagutelli, Xavier AU - Vaiman, Daniel PY - 2007/07/01/ UR - http://dx.doi.org/10.1534/genetics.107.072157 ER -