Overexpression of Shp2 tyrosine phosphatase is implicated in leukemogenesis in adult human leukemia.

@article{citeulike:2739313, abstract = {Shp2 tyrosine phosphatase plays a critical role in hematopoiesis, and dominant active mutations have been detected in the human gene PTPN11, encoding Shp2, in child leukemia patients. We report here that although no such mutations were detected in 44 adult leukemia patients screened, Shp2 expression levels were significantly elevated in primary leukemia cells and leukemia cell lines, as compared with normal hematopoietic progenitor cells. The Shp2 protein amounts correlated well with the hyperproliferative capacity but were inversely associated with the differentiation degree of leukemia cells. Suppression of Shp2 expression induced apoptosis and inhibition of leukemic cell clonogenic growth. Notably, the majority of Shp2 was preferentially localized to the plasma membrane and was constitutively phosphorylated on tyrosine in leukemia cells, and also in normal hematopoietic cells following mitogenic stimulation. Based on these results, we propose that aberrantly increased expression of Shp2 may contribute, collaboratively with other factors, to leukemogenesis.}, address = {Department of Hematology, Second Affiliated Hospital, School of Medicine, Cancer Institute, Zhejiang University, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310009, China. xurongzhen@hzcnc.com}, author = {Xu, R. and Yu, Y. and Zheng, S. and Zhao, X. and Dong, Q. and He, Z. and Liang, Y. and Lu, Q. and Fang, Y. and Gan, X. and Xu, X. and Zhang, S. and Dong, Q. and Zhang, X. and Feng, G. S. }, citeulike-article-id = {2739313}, doi = {10.1182/blood-2004-10-4057}, issn = {0006-4971}, journal = {Blood}, keywords = {shp2}, month = {November}, number = {9}, pages = {3142--3149}, posted-at = {2008-04-30 18:48:08}, priority = {2}, title = {Overexpression of Shp2 tyrosine phosphatase is implicated in leukemogenesis in adult human leukemia.}, url = {http://dx.doi.org/10.1182/blood-2004-10-4057}, volume = {106}, year = {2005} }

TY - JOUR ID - citeulike:2739313 L3 - citeulike-article-id:2739313 TI - Overexpression of Shp2 tyrosine phosphatase is implicated in leukemogenesis in adult human leukemia. JF - Blood VL - 106 IS - 9 SP - 3142 EP - 3149 AD - Department of Hematology, Second Affiliated Hospital, School of Medicine, Cancer Institute, Zhejiang University, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310009, China. xurongzhen@hzcnc.com SN - 0006-4971 N2 - Shp2 tyrosine phosphatase plays a critical role in hematopoiesis, and dominant active mutations have been detected in the human gene PTPN11, encoding Shp2, in child leukemia patients. We report here that although no such mutations were detected in 44 adult leukemia patients screened, Shp2 expression levels were significantly elevated in primary leukemia cells and leukemia cell lines, as compared with normal hematopoietic progenitor cells. The Shp2 protein amounts correlated well with the hyperproliferative capacity but were inversely associated with the differentiation degree of leukemia cells. Suppression of Shp2 expression induced apoptosis and inhibition of leukemic cell clonogenic growth. Notably, the majority of Shp2 was preferentially localized to the plasma membrane and was constitutively phosphorylated on tyrosine in leukemia cells, and also in normal hematopoietic cells following mitogenic stimulation. Based on these results, we propose that aberrantly increased expression of Shp2 may contribute, collaboratively with other factors, to leukemogenesis. KW - shp2 AU - Xu, R AU - Yu, Y AU - Zheng, S AU - Zhao, X AU - Dong, Q AU - He, Z AU - Liang, Y AU - Lu, Q AU - Fang, Y AU - Gan, X AU - Xu, X AU - Zhang, S AU - Dong, Q AU - Zhang, X AU - Feng, GS PY - 2005/11/01/ UR - http://dx.doi.org/10.1182/blood-2004-10-4057 ER -