Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules

@article{citeulike:1060282, abstract = {Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells. Here we show an alternative method that harnesses the self-organizing ability of testicular somatic cells. The testicular cells of embryonic or neonatal mice or rats and of newborn pigs were dissociated into single cells. Each of them reorganized into a tubular structure following implantation into the subcutis of immunodeficient mice. When mouse germline stem (GS) cells derived from spermatogonial stem cells and expanded in culture were intermingled with testicular cells of rodents, they were integrated in the reconstituted tubules and differentiated beyond meiosis into spermatids. Normal offspring were produced by the microinjection of those spermatids into oocytes. This method could be applicable to various mammalian species and useful for producing functional gametes from GS cells in a xenoectopic environment. 10.1095/biolreprod.106.056895}, author = {Kita, Kaoru and Watanabe, Takeshi and Ohsaka, Kimito and Hayashi, Hirofumi and Kubota, Yoshinobu and Nagashima, Yoji and Aoki, Ichiro and Taniguchi, Hideki and Noce, Toshiaki and Inoue, Kimiko and Miki, Hiromi and Ogonuki, Narumi and Tanaka, Hiromitsu and Ogura, Atsuo and Ogawa, Takehiko }, citeulike-article-id = {1060282}, doi = {http://dx.doi.org/10.1095/biolreprod.106.056895}, journal = {Biol Reprod}, keywords = {sperm, stem-cell}, month = {February}, number = {2}, pages = {211--217}, posted-at = {2007-01-22 16:31:41}, priority = {2}, title = {Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules}, url = {http://dx.doi.org/10.1095/biolreprod.106.056895}, volume = {76}, year = {2007} }

TY - JOUR ID - citeulike:1060282 L3 - citeulike-article-id:1060282 TI - Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules JF - Biol Reprod VL - 76 IS - 2 SP - 211 EP - 217 N2 - Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells. Here we show an alternative method that harnesses the self-organizing ability of testicular somatic cells. The testicular cells of embryonic or neonatal mice or rats and of newborn pigs were dissociated into single cells. Each of them reorganized into a tubular structure following implantation into the subcutis of immunodeficient mice. When mouse germline stem (GS) cells derived from spermatogonial stem cells and expanded in culture were intermingled with testicular cells of rodents, they were integrated in the reconstituted tubules and differentiated beyond meiosis into spermatids. Normal offspring were produced by the microinjection of those spermatids into oocytes. This method could be applicable to various mammalian species and useful for producing functional gametes from GS cells in a xenoectopic environment. 10.1095/biolreprod.106.056895 KW - sperm KW - stem-cell AU - Kita, Kaoru AU - Watanabe, Takeshi AU - Ohsaka, Kimito AU - Hayashi, Hirofumi AU - Kubota, Yoshinobu AU - Nagashima, Yoji AU - Aoki, Ichiro AU - Taniguchi, Hideki AU - Noce, Toshiaki AU - Inoue, Kimiko AU - Miki, Hiromi AU - Ogonuki, Narumi AU - Tanaka, Hiromitsu AU - Ogura, Atsuo AU - Ogawa, Takehiko PY - 2007/02/01/ UR - http://dx.doi.org/10.1095/biolreprod.106.056895 ER -