PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements.

@article{citeulike:2238767, abstract = {PCR primers for the detection of materials derived from ruminants, pigs, and chickens were newly designed on the basis of sequences of the Art2 short interspersed repetitive element (SINE), PRE-1 SINE, and CR1 long interspersed repetitive element (LINE), respectively. These primers amplified the SINE or LINE from total DNA extracted from the target animals and from test feed containing commercial meat and bone meal (MBM). With the primers, detection of Art2, PRE-1, or CR1 in test feed at concentrations of 0.01\% MBM or less was possible. This method was suitable for the detection of microcontamination of feed by animal materials.}, address = {National Institute of Livestock and Grassland Science, P.O. Box 5, Tsukuba, Norindanchi, Ibaraki 305-0901, Japan. ktajima@affrc.go.jp}, author = {Tajima, K. and Enishi, O. and Amari, M. and Mitsumori, M. and Kajikawa, H. and Kurihara, M. and Yanai, S. and Matsui, H. and Yasue, H. and Mitsuhashi, T. and Kawashima, T. and Matsumoto, M. }, citeulike-article-id = {2238767}, issn = {0916-8451}, journal = {Biosci Biotechnol Biochem}, keywords = {forensic, forensic-animal, species-identification}, month = {October}, number = {10}, pages = {2247--2250}, posted-at = {2008-01-16 11:21:40}, priority = {2}, title = {PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12450143}, volume = {66}, year = {2002} }

TY - JOUR ID - citeulike:2238767 L3 - citeulike-article-id:2238767 TI - PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements. JF - Biosci Biotechnol Biochem VL - 66 IS - 10 SP - 2247 EP - 2250 AD - National Institute of Livestock and Grassland Science, P.O. Box 5, Tsukuba, Norindanchi, Ibaraki 305-0901, Japan. ktajima@affrc.go.jp SN - 0916-8451 N2 - PCR primers for the detection of materials derived from ruminants, pigs, and chickens were newly designed on the basis of sequences of the Art2 short interspersed repetitive element (SINE), PRE-1 SINE, and CR1 long interspersed repetitive element (LINE), respectively. These primers amplified the SINE or LINE from total DNA extracted from the target animals and from test feed containing commercial meat and bone meal (MBM). With the primers, detection of Art2, PRE-1, or CR1 in test feed at concentrations of 0.01% MBM or less was possible. This method was suitable for the detection of microcontamination of feed by animal materials. KW - forensic KW - forensic-animal KW - species-identification AU - Tajima, K AU - Enishi, O AU - Amari, M AU - Mitsumori, M AU - Kajikawa, H AU - Kurihara, M AU - Yanai, S AU - Matsui, H AU - Yasue, H AU - Mitsuhashi, T AU - Kawashima, T AU - Matsumoto, M PY - 2002/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/12450143 ER -