Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites

by: Rickard Sandberg, Joel R Neilson, Arup Sarma, Phillip A Sharp, Christopher B Burge

Science, Vol. 320, No. 5883. (20 June 2008), pp. 1643-1647.

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Résumé

Messenger RNA (mRNA) stability, localization, and translation are largely determined by sequences in the 3' untranslated region (3'UTR). We found a conserved increase in expression of mRNAs terminating at upstream polyadenylation sites after activation of primary murine CD4+ T lymphocytes. This program, resulting in shorter 3'UTRs, is a characteristic of gene expression during immune cell activation and correlates with proliferation across diverse cell types and tissues. Forced expression of full-length 3'UTRs conferred reduced protein expression. In some cases the reduction in protein expression could be reversed by deletion of predicted microRNA target sites in the variably included region. Our data indicate that gene expression is coordinately regulated, such that states of increased proliferation are associated with widespread reductions in the 3'UTR-based regulatory capacity of mRNAs. 10.1126/science.1155390

@article{citeulike:2910867, abstract = {Messenger RNA (mRNA) stability, localization, and translation are largely determined by sequences in the 3' untranslated region (3'UTR). We found a conserved increase in expression of mRNAs terminating at upstream polyadenylation sites after activation of primary murine CD4+ T lymphocytes. This program, resulting in shorter 3'UTRs, is a characteristic of gene expression during immune cell activation and correlates with proliferation across diverse cell types and tissues. Forced expression of full-length 3'UTRs conferred reduced protein expression. In some cases the reduction in protein expression could be reversed by deletion of predicted microRNA target sites in the variably included region. Our data indicate that gene expression is coordinately regulated, such that states of increased proliferation are associated with widespread reductions in the 3'UTR-based regulatory capacity of mRNAs. 10.1126/science.1155390}, author = {Sandberg, Rickard and Neilson, Joel R. and Sarma, Arup and Sharp, Phillip A. and Burge, Christopher B. }, citeulike-article-id = {2910867}, doi = {http://dx.doi.org/10.1126/science.1155390}, journal = {Science}, keywords = {mir, mirna, mrna, utr}, month = {June}, number = {5883}, pages = {1643--1647}, posted-at = {2008-07-01 11:55:45}, priority = {2}, title = {Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites}, url = {http://dx.doi.org/10.1126/science.1155390}, volume = {320}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2910867 L3 - citeulike-article-id:2910867 TI - Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites JF - Science VL - 320 IS - 5883 SP - 1643 EP - 1647 N2 - Messenger RNA (mRNA) stability, localization, and translation are largely determined by sequences in the 3' untranslated region (3'UTR). We found a conserved increase in expression of mRNAs terminating at upstream polyadenylation sites after activation of primary murine CD4+ T lymphocytes. This program, resulting in shorter 3'UTRs, is a characteristic of gene expression during immune cell activation and correlates with proliferation across diverse cell types and tissues. Forced expression of full-length 3'UTRs conferred reduced protein expression. In some cases the reduction in protein expression could be reversed by deletion of predicted microRNA target sites in the variably included region. Our data indicate that gene expression is coordinately regulated, such that states of increased proliferation are associated with widespread reductions in the 3'UTR-based regulatory capacity of mRNAs. 10.1126/science.1155390 KW - mir KW - mirna KW - mrna KW - utr AU - Sandberg, Rickard AU - Neilson, Joel AU - Sarma, Arup AU - Sharp, Phillip AU - Burge, Christopher PY - 2008/06/20/ UR - http://dx.doi.org/10.1126/science.1155390 ER -

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