High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.

by: SL Spurgeon, RC Jones, R Ramakrishnan

PLoS ONE, Vol. 3, No. 2. (2008)

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Résumé

We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

@article{citeulike:3008715, abstract = {We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.}, address = {Fluidigm Corporation, South San Francisco, California, USA.}, author = {Spurgeon, S. L. and Jones, R. C. and Ramakrishnan, R. }, citeulike-article-id = {3008715}, doi = {http://dx.doi.org/10.1371/journal.pone.0001662}, issn = {1932-6203}, journal = {PLoS ONE}, keywords = {microfluidics, rtpcr}, number = {2}, posted-at = {2008-07-16 16:12:50}, priority = {2}, title = {High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.}, url = {http://dx.doi.org/10.1371/journal.pone.0001662}, volume = {3}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:3008715 L3 - citeulike-article-id:3008715 TI - High throughput gene expression measurement with real time PCR in a microfluidic dynamic array. JF - PLoS ONE VL - 3 IS - 2 AD - Fluidigm Corporation, South San Francisco, California, USA. SN - 1932-6203 N2 - We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays. KW - microfluidics KW - rtpcr AU - Spurgeon, SL AU - Jones, RC AU - Ramakrishnan, R PY - 2008/// UR - http://dx.doi.org/10.1371/journal.pone.0001662 ER -

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