Bio-distribution and metabolic paths of silica coated CdSeS quantum dots

@article{citeulike:3101421, abstract = {With the rapid development of quantum dot (QD) technology, water-soluble QDs have the prospect of being used as a biological probe for specific diagnoses, but their biological behaviors in vivo are little known. Our recent in vivo studies concentrated on the bio-kinetics of QDs coated by hydroxyl group modified silica networks (the QDs are 21.3 ± 2.0 nm in diameter and have maximal emission at 570 nm). Male ICR mice were intravenously given the water-soluble QDs with a single dose of 5 nmol/mouse. Inductively coupled plasma-mass spectrometry was used to measure the 111Cd content to indicate the concentration of QDs in plasma, organs, and excretion samples collected at predetermined time intervals. Meanwhile, the distribution and aggregation state of QDs in tissues were also investigated by pathological examination and differential centrifugation. The plasma half-life and clearance of QDs were 19.8 ± 3.2 h and 57.3 ± 9.2 ml/h/kg, respectively. The liver and kidney were the main target organs for QDs. The QDs metabolized in three paths depending on their distinct aggregated states in vivo. A fraction of free QDs, maintaining their original form, could be filtered by glomerular capillaries and excreted via urine as small molecules within five days. Most QDs bound to protein and aggregated into larger particles that were metabolized in the liver and excreted via feces in vivo. After five days, 8.6\% of the injected dose of aggregated QDs still remained in hepatic tissue and it was difficult for this fraction to clear.}, author = {Chen, Zhen and Chen, Hu and Meng, Huan and Xing, Gengmei and Gao, Xueyun and Sun, Baoyun and Shi, Xiaoli and Yuan, Hui and Zhang, Chengcheng and Liu, Ru and Zhao, Feng and Zhao, Yuliang and Fang, Xiaohong }, citeulike-article-id = {3101421}, doi = {http://dx.doi.org/10.1016/j.taap.2008.03.022}, journal = {Toxicology and Applied Pharmacology}, keywords = {biodistribution, fluorescence, murine-model, pharmacokinetic, quantum-dot}, month = {August}, number = {3}, pages = {364--371}, posted-at = {2008-08-08 17:25:12}, priority = {2}, title = {Bio-distribution and metabolic paths of silica coated CdSeS quantum dots}, url = {http://dx.doi.org/10.1016/j.taap.2008.03.022}, volume = {230}, year = {2008} }

TY - JOUR ID - citeulike:3101421 L3 - citeulike-article-id:3101421 TI - Bio-distribution and metabolic paths of silica coated CdSeS quantum dots JF - Toxicology and Applied Pharmacology VL - 230 IS - 3 SP - 364 EP - 371 N2 - With the rapid development of quantum dot (QD) technology, water-soluble QDs have the prospect of being used as a biological probe for specific diagnoses, but their biological behaviors in vivo are little known. Our recent in vivo studies concentrated on the bio-kinetics of QDs coated by hydroxyl group modified silica networks (the QDs are 21.3 ± 2.0 nm in diameter and have maximal emission at 570 nm). Male ICR mice were intravenously given the water-soluble QDs with a single dose of 5 nmol/mouse. Inductively coupled plasma-mass spectrometry was used to measure the 111Cd content to indicate the concentration of QDs in plasma, organs, and excretion samples collected at predetermined time intervals. Meanwhile, the distribution and aggregation state of QDs in tissues were also investigated by pathological examination and differential centrifugation. The plasma half-life and clearance of QDs were 19.8 ± 3.2 h and 57.3 ± 9.2 ml/h/kg, respectively. The liver and kidney were the main target organs for QDs. The QDs metabolized in three paths depending on their distinct aggregated states in vivo. A fraction of free QDs, maintaining their original form, could be filtered by glomerular capillaries and excreted via urine as small molecules within five days. Most QDs bound to protein and aggregated into larger particles that were metabolized in the liver and excreted via feces in vivo. After five days, 8.6% of the injected dose of aggregated QDs still remained in hepatic tissue and it was difficult for this fraction to clear. KW - biodistribution KW - fluorescence KW - murine-model KW - pharmacokinetic KW - quantum-dot AU - Chen, Zhen AU - Chen, Hu AU - Meng, Huan AU - Xing, Gengmei AU - Gao, Xueyun AU - Sun, Baoyun AU - Shi, Xiaoli AU - Yuan, Hui AU - Zhang, Chengcheng AU - Liu, Ru AU - Zhao, Feng AU - Zhao, Yuliang AU - Fang, Xiaohong PY - 2008/08/01/ UR - http://dx.doi.org/10.1016/j.taap.2008.03.022 ER -