The emergence of automated high-frequency flow cytometry: revealing temporal and spatial phytoplankton variability

@article{citeulike:2725360, abstract = {Phytoplankton observation is the product of a number of trade-offs related to sampling processes, required level of diversity and size spectrum analysis capabilities of the techniques involved. Instruments combining the morphological and high-frequency analysis for phytoplankton cells are now available. This paper presents an application of the automated high-resolution flow cytometer Cytosub as a tool for analysing phytoplanktonic cells in their natural environment. High resolution data from a temporal study in the Bay of Marseille (analysis every 30 min over 1 month) and a spatial study in the Southern Indian Ocean (analysis every 5 min at 10 knots over 5 days) are presented to illustrate the capabilities and limitations of the instrument. Automated high-frequency flow cytometry revealed the spatial and temporal variability of phytoplankton in the size range 1-[~]50 {micro}m that could not be resolved otherwise. Due to some limitations (instrumental memory, volume analysed per sample), recorded counts could be statistically too low. By combining high-frequency consecutive samples, it is possible to decrease the counting error, following Poisson's law, and to retain the main features of phytoplankton variability. With this technique, the analysis of phytoplankton variability combines adequate sampling frequency and effective monitoring of community changes. 10.1093/plankt/fbn005}, author = {Thyssen, Melilotus and Tarran, Glen A. and Zubkov, Mikhail V. and Holland, Ross J. and Gregori, Gerald and Burkill, Peter H. and Denis, Michel }, citeulike-article-id = {2725360}, doi = {10.1093/plankt/fbn005}, journal = {J. Plankton Res.}, keywords = {fcm}, month = {March}, number = {3}, pages = {333--343}, posted-at = {2008-05-05 03:52:47}, priority = {2}, title = {The emergence of automated high-frequency flow cytometry: revealing temporal and spatial phytoplankton variability}, url = {http://dx.doi.org/10.1093/plankt/fbn005}, volume = {30}, year = {2008} }

TY - JOUR ID - citeulike:2725360 L3 - citeulike-article-id:2725360 TI - The emergence of automated high-frequency flow cytometry: revealing temporal and spatial phytoplankton variability JF - J. Plankton Res. VL - 30 IS - 3 SP - 333 EP - 343 N2 - Phytoplankton observation is the product of a number of trade-offs related to sampling processes, required level of diversity and size spectrum analysis capabilities of the techniques involved. Instruments combining the morphological and high-frequency analysis for phytoplankton cells are now available. This paper presents an application of the automated high-resolution flow cytometer Cytosub as a tool for analysing phytoplanktonic cells in their natural environment. High resolution data from a temporal study in the Bay of Marseille (analysis every 30 min over 1 month) and a spatial study in the Southern Indian Ocean (analysis every 5 min at 10 knots over 5 days) are presented to illustrate the capabilities and limitations of the instrument. Automated high-frequency flow cytometry revealed the spatial and temporal variability of phytoplankton in the size range 1-[~]50 {micro}m that could not be resolved otherwise. Due to some limitations (instrumental memory, volume analysed per sample), recorded counts could be statistically too low. By combining high-frequency consecutive samples, it is possible to decrease the counting error, following Poisson's law, and to retain the main features of phytoplankton variability. With this technique, the analysis of phytoplankton variability combines adequate sampling frequency and effective monitoring of community changes. 10.1093/plankt/fbn005 KW - fcm AU - Thyssen, Melilotus AU - Tarran, Glen AU - Zubkov, Mikhail AU - Holland, Ross AU - Gregori, Gerald AU - Burkill, Peter AU - Denis, Michel PY - 2008/03/01/ UR - http://dx.doi.org/10.1093/plankt/fbn005 ER -