A Genome-wide RNA Interference Screen Reveals that Variant Histones Are Necessary for Replication-Dependent Histone Pre-mRNA Processing

@article{citeulike:2084668, abstract = {Summary Metazoan replication-dependent histone mRNAs are not polyadenylated and instead end in a conserved stem loop that is the cis element responsible for coordinate posttranscriptional regulation of these mRNAs. Using biochemical approaches, only a limited number of factors required for cleavage of histone pre-mRNA have been identified. We therefore performed a genome-wide RNA interference screen in Drosophila cells using a GFP reporter that is expressed only when histone pre-mRNA processing is disrupted. Four of the 24 genes identified encode proteins also necessary for cleavage/polyadenylation, indicating mechanistic conservation in formation of different mRNA 3' ends. We also unexpectedly identified the histone variants H2Av and H3.3A/B. In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the histone locus, suggesting there is a regulatory pathway that coordinates the production of variant and canonical histones that acts via localization of essential histone pre-mRNA processing factors.}, author = {Wagner, Eric J. and Burch, Brandon D. and Godfrey, Ashley C. and Salzler, Harmony R. and Duronio, Robert J. and Marzluff, William F. }, citeulike-article-id = {2084668}, doi = {http://dx.doi.org/10.1016/j.molcel.2007.10.009}, journal = {Molecular Cell}, keywords = {genome, histone, rna}, month = {November}, number = {4}, pages = {692--699}, posted-at = {2007-12-10 05:03:46}, priority = {1}, title = {A Genome-wide RNA Interference Screen Reveals that Variant Histones Are Necessary for Replication-Dependent Histone Pre-mRNA Processing}, url = {http://dx.doi.org/10.1016/j.molcel.2007.10.009}, volume = {28}, year = {2007} }

TY - JOUR ID - citeulike:2084668 L3 - citeulike-article-id:2084668 TI - A Genome-wide RNA Interference Screen Reveals that Variant Histones Are Necessary for Replication-Dependent Histone Pre-mRNA Processing JF - Molecular Cell VL - 28 IS - 4 SP - 692 EP - 699 N2 - Summary Metazoan replication-dependent histone mRNAs are not polyadenylated and instead end in a conserved stem loop that is the cis element responsible for coordinate posttranscriptional regulation of these mRNAs. Using biochemical approaches, only a limited number of factors required for cleavage of histone pre-mRNA have been identified. We therefore performed a genome-wide RNA interference screen in Drosophila cells using a GFP reporter that is expressed only when histone pre-mRNA processing is disrupted. Four of the 24 genes identified encode proteins also necessary for cleavage/polyadenylation, indicating mechanistic conservation in formation of different mRNA 3' ends. We also unexpectedly identified the histone variants H2Av and H3.3A/B. In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the histone locus, suggesting there is a regulatory pathway that coordinates the production of variant and canonical histones that acts via localization of essential histone pre-mRNA processing factors. KW - genome KW - histone KW - rna AU - Wagner, Eric AU - Burch, Brandon AU - Godfrey, Ashley AU - Salzler, Harmony AU - Duronio, Robert AU - Marzluff, William PY - 2007/11/30/ UR - http://dx.doi.org/10.1016/j.molcel.2007.10.009 ER -